Figure 5. Ezh2 accumulates at Fok-1 induced DSBs. U2OS cells stably expressing the reporter locus were transfected with wild-type Fok-1 (FOK1 WT) or Fok1 catalytically inactive mutant (FOK1 mut) endonuclease. Cells were then fixed with 1% paraformaldehyde 18m h after transfection and harvested for chromatin immunoprecipitation (Ch-IP). Ch-IP was performed with Ezh2, γ-H2AX, and IgG antibodies. Quantitative PCR using 5 representative primers was done, and the Fok-1 DSB induced enrichment for each primer and antibody is plotted.