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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Invest New Drugs. 2013 Jul 26;32(1):68–74. doi: 10.1007/s10637-013-9987-y

Fig.4.

Fig.4

Disruption of Hsp90-Cdc37 in Panc-1 cells by withaferin A (WA) and its analogues. A. Immunoprecipitation of Hsp90 and Cdc37 complex. Cell lysates (500 μg total protein) were immunoprecipitated with Hsp90 antibody. Western blot was performed to detect Cdc37 and Hsp90 using specific antibodies. Panc-1 cells were treated with 20μM WA, WE, HWE, AzWA or WP for 24 h, respectively. Lysis, total cell lysate; IgG, without antibody. B. Total expression levels of Cdc37 in Panc-1 cells. Panc-1 cells were treated with WA, WE, HWE, AzWA or WP for 24 h, respectively. Equal amounts of protein (50μg/lane) were subjected to SDS-PAGE and analyzed by Western blot with specific antibodies to Cdc37 and Actin. Actin was served as internal standard. Results are representative of three independent experiments.