Table 1. Universal primers for 16S and CO1 genes with associated blocking primers.
Primers | Sequence 5'-3' | Reference |
---|---|---|
16S1F | GACGAKAAGACCCTA | [12] |
Herring Blocker | GACCCTATGGAGCTTTAGACGCCCAC3 | This study |
16S2R | CGCTGTTATCCCTADRGTAACT | [12] |
Puffin Blocker | CCCTGGGGTAGCTTGGTCCATTGATCC3 | This study |
Uni-MinibarF1 | TCCACTAATCACAARGATATTGGTAC | [33] |
Uni-MinibarR1 | GAAAATCATAATGAAGGCATGAGC | [33] |
Italics on 5’ region of blocking primers depict area of overlap on 3’ end of universal primer.