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. 2013 Dec 16;8(12):e82600. doi: 10.1371/journal.pone.0082600

Figure 1. Far-western immunoblot analysis demonstrating that only the N-terminal of recombinant epididymal protease inhibitor (N-rEppin) and rEppin can binds to reducing seminal plasma proteins.

Figure 1

A The monomer and multimers of rEppin, N-rEppin, C-rEppin in Western-blotting; N-Ep: N-rEppin (Eppin18-76Mr 10000); Ep: rEppin (Mr 18000); C-Ep: C-rEppin (Eppin75-133Mr 12000); B: Amido Black Staining of seminal plasma proteins(Stain the membrane for 5 minites with Amido Black, rinse the stained membrane for 30 to 45 sec with TBST three times),; lane 1: Non-reducing seminal plasma proteins, lane 2-4: Reducing seminal plasma proteins; M: marker; C: M: marker; lane 5-6: lane 1-2 (B) incubated with rEppin and probed with anti-his tag; lane 7: lane3 (B) incubated with C-rEppin and probed with anti-his tag; lane 8: lane 4(B) incubated with N-rEppin and probed with anti-his tag.