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. 2013 Dec 16;8(12):e82600. doi: 10.1371/journal.pone.0082600

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© 2013 zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Seminal vesicle fluid was separated by 2-D electrophoresis\ and was stained overnight with 0.01% Bio-Rad R-250 Coomassie in 10% acetic acid.

B. Far Western blot: Incubate the membrane with 6-His-Eppin protein and probed with anti-His. We can see the positive spot molecular weight 55-72KDa on the blot (arrowhead).