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. 2013 Dec 16;8(12):e82600. doi: 10.1371/journal.pone.0082600

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© 2013 zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A Phase contrast image of spermatozoon.

B. Immunolocalization of Eppin on the head and tail of ejaculate human spermatozoa with anti-Eppin antibodies and detected with fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit antibodies (green).

C. Immunolocalization of Fn on the head and tail of ejaculate human spermatozoa with anti-Fn antibodies and detected with Cy3 (cyanine-3)-conjugated goat anti-mouse antibodies (red).

D. Dual-immunofluorescence staining of Fn (red) and Eppin (green) in human sperm showing that the strong colocalization (→) is seen in the postacrosomal and midpiece region of the head (yellow areas).

E. Control exposure with fluorescein isothiocyanate (FITC) conjugated goat anti-rabbit IgG and Cy3 (cyanine-3)-conjugated anti-mouse IgG, with isotype mouse antibody and rabbit antibody instead of primary antibody.