Table 7. Results of ranking of nine candidate reference genes obtained using six different software programs and rank aggregation of candidate reference genes for longissimus muscle.
| Rank Position | GenEx (NormFinder) | GenEx (geNorm) | NormFinder | BestKeeper | geNorm | qBasePlus | Consensus |
|---|---|---|---|---|---|---|---|
| 1 | ACAC | ALAS | ACAC | ACAC | HMBS | HSP-90 | ACAC |
| 2 | HSP-90 | GAPDH | HSP-90 | B2M | GAPDH | ACAC | HSP-90 |
| 3 | B2M | ACTB | B2M | HSP-90 | ALAS | ALAS | ALAS |
| 4 | ALAS | HMBS | ALAS | 18s rRNA | HSP-90 | HMBS | B2M |
| 5 | HMBS | ACAC | HMBS | ALAS | ACAC | GAPDH | HMBS |
| 6 | 18s rRNA | HSP-90 | 18s rRNA | HMBS | 18s rRNA | 18s rRNA | 18s rRNA |
| 7 | GAPDH | B2M | GAPDH | GAPDH | B2M | B2M | GAPDH |
| 8 | ACTB | 18s rRNA | ACTB | ACTB | ACTB | ACTB | ACTB |
| 9 | RPS18 | RPS18 | RPS18 | RPS18 | RPS18 | RPS18 | RPS18 |