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. 2013 Dec 16;8(12):e83183. doi: 10.1371/journal.pone.0083183

Figure 4. Inhibition of JNK restores TNFα-mediated reduction of adipolin expression in 3T3-L1 adipocytes.

Figure 4

The mRNA levels of adipolin (APL) and KLF15 were measured by quantitative RT-PCR method. A and B, Effect of JNK inhibitor on mRNA levels of APL (A) and KLF15 (B) in adipocytes. 3T3-L1 adipocytes were cultured in the presence or absence of JNK inhibitor, SP600125 (10 µM) for 1 h followed by treatment with TNFα (10 ng/ml) or vehicle for 24 h. N = 3 in each group. C, Effect of p38 MAPK inhibitor on APL mRNA levels in adipocytes. 3T3-L1 adipocytes were treated with or without p38 MAPK inhibitor, SB203580 (10 µM) for 1 h followed by stimulation with TNFα (10 ng/ml) or vehicle for 24 h. N = 3 in each group.