Table 1. Seawater chemistry for different experiments on Acanthaster planci.
Experiment/Nominal Target pHNBS | N | pH [NBS/derived] | AT [µmol/kg] | DIC [µmol/kg] | pCO2 [µatm] | ΩCa | ΩAr | |
Fertilisation: | 8.1 | 1 | 8.11 | 2349 | 2071 | 520 | 4.89 | 3.25 |
7.9 | 1 | 7.91 | 2353 | 2174 | 877 | 3.40 | 2.26 | |
7.6 | 1 | 7.67 | 2356 | 2278 | 1658 | 2.06 | 1.37 | |
Sperm: | 8.1 | 2 | 8.11 (8.11–8.11) | 2333 (1) | 2050 (0) | 499 (7) | 4.96 (0.02) | 3.03 (0.01) |
7.9 | 2 | 7.84 (7.84–7.84) | 2337 (1) | 2190 (0) | 1053 (7) | 2.92 (0.02) | 1.94 (0.01) | |
7.6 | 2 | 7.61 (7.61–7.61) | 2339 (1) | 2281 (2) | 1892 (26) | 1.83 (0.02) | 1.21 (0.01) | |
Larvae: | 8.1 | 10 | 8.09 (8.05–8.12) | 2329 (15) | 2062 (21) | 536 (60) | 4.68 (0.34) | 3.11 (0.22) |
7.8 | 10 | 7.78 (7.76–7.80) | 2329 (15) | 2207 (17) | 1211 (36) | 4.02 (1.02) | 2.67 (0.68) | |
7.6 | 10 | 7.57 (7.55–7.59) | 2329 (15) | 2284 (14) | 2056 (12) | 3.36 (1.02) | 2.24 (0.68) | |
CCA: Pre-Ind. | 8.2 | 7 | 8.23 (8.18–8.29) | 2337 (16) | 1995 (27) | 363 (56) | 5.85 (0.62) | 3.88 (0.42) |
Present | 8.1 | 7 | 8.15 (8.13–8.16) | 2337 (10) | 2047 (14) | 458 (18) | 5.03 (0.22) | 3.33 (0.16) |
8.0 | 7 | 8.05 (8.04–8.11) | 2342 (12) | 2100 (28) | 598 (84) | 4.25 (0.25) | 2.81 (0.16) | |
7.9 | 7 | 7.94 (7.89–8.00) | 2339 (12) | 2158 (22) | 806 (111) | 3.44 (0.24) | 2.28 (0.16) | |
Settlement: Pre-Ind. | 8.2 | 1 | 8.25 | 2347 | 1979 | 341 | 6.31 | 4.20 |
Present | 8.1 | 1 | 8.14 | 2345 | 2047 | 471 | 5.19 | 3.46 |
8.0 | 1 | 8.04 | 2350 | 2104 | 609 | 4.40 | 2.93 | |
7.9 | 1 | 7.95 | 2350 | 2151 | 783 | 3.69 | 2.45 |
Nominal target pH represents the pH value used to denominate treatments throughout the text. pHderived is accurate pH value as derived from DIC and AT measurements; all pH values are given on the NBS scale. Upper and lower 95% confidence intervals for pH and standard deviations for all other parameters are shown in parentheses. pCO2, calcite saturation state (ΩCa) and aragonite saturation state (ΩAr) were calculated based on measured total alkalinity (AT) and dissolved inorganic carbon (DIC). N = number of samples. Pre-Ind.: Pre-Industrial treatment. Sperm: experiments on sperm velocity and percent of motile sperm; larvae: experiment on larval development, CCA: conditions during crustose coralline algal culture.