Table 3. Results of mixed model analysis of variance for sperm velocity and percentage of motile sperm (% motile) of A. planci sperm subjected to three different pH/pCO2 conditions (factor pH).
Velocity | % Motile | ||||||
DF | MS | F | p | MS | F | p | |
pH | 2 | 0.0218 | 23.57 | <0.0001 | 1.2600 | 23.86 | <0.0001 |
Male | 5 | 0.0381 | 41.23 | <0.0001 | 0.4400 | 8.33 | <0.0001 |
pH X M | 10 | 0.0015 | 1.58 | 0.1558 | 0.0531 | 1.01 | 0.4588 |
Slide (pH X M) | 34 | 0.0009 | 1.99 | <0.0001 | 0.0528 | 8.32 | <0.0001 |
Residual | 232 | 0.0005 | 0.0063 | ||||
Total | 283 |
Sperm was derived from 6 separate males (Males). Slide is a random/nested factor representing measurements taken from three independent dilutions for each male x pH combination. Velocity data were log-transformed, motility data arcsine-square root-transformed for analysis.