Skip to main content
. 2013 Dec 16;8(12):e84107. doi: 10.1371/journal.pone.0084107

Figure 4. Expression of pro-inflammatory genes induced by LPS is inhibited by Compound 1.

Figure 4

Peritoneal macrophages were treated with compound 1 (25 μM) and stimulated with LPS (1 μg/mL). After 180 min the RNA was isolated following the Trizol method. The amount of mRNA for TNF-α (A), IL-6 (B), IP-10 (C), IL-1β (D), iNOS (E), COX-2 (F) and MCP-1 (G) was determined by real-time RT-PCR. Results were normalized to HPRT expression and are presented as fold induction of mRNA expression relative to control samples. Results represent means ± S.E.M. from stimuli performed in duplicates and are representative of two different experiments. *, P ˂ 0.05; **, P ˂ 0.01, compared with LPS stimulus alone.