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. 2013 Dec 16;8(12):e84172. doi: 10.1371/journal.pone.0084172

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© 2013 Yi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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All of TM4 and its site mutants (TM4/W775A, TM4/W830A, and TM4/W775A/W830A) were expressed in E. coli. The experiment was performed by incubating 0.2 µM of the purified TM1, TM3, TM4 or the site-directed mutants of TM4 respectively with 10 mg ml^-1 Avicel in citrate buffer (pH 6.0) at 75°C for 13 h.