Figure 5. Hydrolysis of Avicel, Filter paper and PASC by CbCel9A/Cel48A, its truncational mutants, and their binary combinations as analyzed by a reducing sugar assay.

The experiments were carried out in citrate buffer (pH 6.0) at 75°C for 16 h by incubating 0.4 µM of each enzyme with 10 mg ml^-1 of Avicel or with 16 discs of Whatman No. 1 filter paper (0.6 cm in diameter) or by incubating 0.2 µM of each enzyme with 2.5 mg ml^-1 of PASC as the substrate. The numbers on top of the columns represent the degrees of synergy calculated as described in Materials and Methods.