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. 2013 Dec 16;8(12):e84172. doi: 10.1371/journal.pone.0084172

Table 1. Kinetic parameters of CbCel9A/Cel48A wild-type and its truncational mutants on cellulosic substrates a .

Enzyme PASC b
Avicel (µM glucose equivalents/min/µM enzyme) Filter paper (µM glucose equivalents/min/µM enzyme)
k cat (s-1) K m (mg ml-1) k cat/K m (s-1 ml mg-1)
WT 2.0±0.3 2.6±1.0 0.8±0.3 22.8±1.3 13.1±0.3
TM1 1.6±0.2 1.8±0.6 0.9±0.3 10.2±1.0 7.0±1.8
TM2 0.2±0.0 1.5±0.6 0.1±0.0 2.2±0.1 1.3±0.2
TM3 5.1±0.3 2.3±0.3 2.2±0.3 3.5±0.7 4.5±0.6

a The reactions were performed in a 50 mM sodium-citrate buffer with 150 mM NaCl (pH6.0) at 75°C.

b PASC: phosphoric acid swollen cellulose.