Figure 2. ROCK1 deficiency does not affect platelet shape change.

Isolated murine (A, B) or human (C) platelets were stimulated with collagen (10 µg/ml,) and aggregation and ATP release (where indicated) were measured in a lumi aggregometer. (A) Wild-type and ROCK1-deficient platelets responded similarly to collagen. (B) Addition of non-specific ROCK inhibitor Y-27632 to ROCK1-deficient platelets abolished platelet shape change, similar to its effect on human platelets, though it did not alter ATP secretion. (C) The traces shown are representative of at least 3 independent experiments.