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. 2013 Dec 16;8(12):e84649. doi: 10.1371/journal.pone.0084649

Figure 3. ROCK inhibition and ROCK1-deficiency increases collagen-induced phosphatidylserine expression and thrombin generation.

Figure 3

Isolated human (A and B) or murine (C) platelets were stimulated with collagen (10 µg/mL), and FITC-lactadherin and PE-labeled anti-CD42b were added. The exposure of phosphatidylserine was analyzed by flow cytometry. Platelets were incubated with Y-27632 or latrunculin A for 20 minutes before collagen. (D) ROCK1-deficient and wild-type murine platelets were activated with collagen in the presence of prothrombin, factor Xa, calcium, and factor V; thrombin generation was measured by the amiodolyis of thrombin substarte S-2238. (n= 3/group). * denotes a p value of < 0.05.