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. 2013 Dec 16;8(12):e84649. doi: 10.1371/journal.pone.0084649

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fura-2 AM loaded platelets were incubated with buffer, Y-27632 and BAPTA-AM as described in materials and method and activated with collagen. The increase in calcium levels was quantified by measuring Fura-2 fluorescence. (A) Representative traces of Ca²⁺ signaling induced by collagen in isolated murine platelets. (B) Group data of intracellular Ca²⁺ levels in resting and activated platelets (n= 3/group) in presence of buffer, Y-27632 and BAPTA-AM. .