Figure 5.

Number of proteins two-fold or more abundant in pairwise comparisons, with other matix treatments equivalent (color bar on x-axis corresponds with Figure 2 design), with protein bars colored by KEGG function. (A) Proteins more abundant with scarce PO₄³⁻ relative to replete conditions and vice versa (right and left bars, respectively). (B) Proteins more abundant with short-term Cd addition relative to no Cd added and vice versa (right and left bars, respectively). (C) Proteins more abundant with scarce Zn relative to replete conditions and vice versa (left and right bars, respectively). Comparison of (A–C) shows that the combination of Zn and PO₄³⁻ scarcity causes the greatest number of proteins to be differentially abundant. (B) shows that the presence of Zn causes less change in number of proteins when Cd is added. Functions and abundances for each protein are in Tables 1–3 and Supplementary Tables 1A,B, and D–J.