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. 2013 Nov 5;8(11):e78374. doi: 10.1371/journal.pone.0078374

Figure 4. Stimulation of CCK promoter activity by DJ-1 along with RREB1.

Figure 4

A–C. D2 cells were transfected with various amounts of expression vectors for DJ-1-HA (A), FLAG-RREB1 (B) and T7-Sp1 (C) along with pGL3-CCK-1615. Forty-eight hrs after transfection, cell extracts were prepared and their luciferase activity was measured as described in Materials and methods. n = 4. D and E. D2 cells were transfected with various amounts of expression vectors for DJ-1-HA along with fixed amount of FLAG-RREB1 (D) or T7-Sp1 (E) and pGL3-CCK-1615. Forty-eight hrs after transfection, cell extracts were prepared and their luciferase activity was measured as described in Materials and methods. n = 4. Statistical analyses were performed using the Tukey-Kramer test.