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. 2013 Nov 19;8(11):e79843. doi: 10.1371/journal.pone.0079843

Figure 5. The four-gene signature is regulated by p53 activity.

Figure 5

Activation of p53 represses the four-gene signature. (A, B) Quantitative PCR demonstrates marked decrease of mRNA levels of the four genes after Nutlin-3a treatment (10 uM for 8 hours) in multiple p53 wild-type neuroblastoma lines (shown here LAN5 and IMR32). However, this effect is completely abrogated after p53 silencing or when the effect of Nutlin-3a is tested in a p53 mutant neuroblastoma cell line (LAN1) (C). Each error bar represents two biological replicates.