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Immunostaining and FISH for minichromosome. (A) Merged image; (B) Green is ZmBs; (C) Red is CENH3, the centromeric histone signals; (D) Blue is DAPI. Arrows indicate that the minichromosome sister chromatids separate in meiosis I and each sister has a functional centromere. Bars = 10 μm.
