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. 2013 Dec 16;210(13):2803–2811. doi: 10.1084/jem.20130678

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© 2013 Vom Berg et al.

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Figure 2. — T cell dependency of IL-12–mediated glioma rejection. (A, B and D, E) GL-261luc:IL-12 cells were implanted i.c. into various immunodeficient mouse strains. Animals were monitored for up to 91 d; Log-Rank test; *, P < 0.05; **, P < 0.01; ***, P < 0.001. (A) Survival of WT, Rag2^−/−Il2rg^−/−, Rag1^−/−, and Il15ra^−/− mice (n ≥ 13/group); p(Rag2^−/−Il2rg^−/− vs. WT), p(Rag1^−/− vs. WT), p(Rag2^−/−Il2rg^−/− vs. Il15ra^−/−) and p(Rag1^−/− vs. Il15ra^−/−) < 0.0001. (B) Survival of WT, β2m^−/− and Ia(b)^−/− mice (n ≥ 12/group); p(β2m^−/− vs. WT) = 0.0052, p(Ia(b)^−/− vs. WT) < 0.0001. (C) Rechallenge of naive WT animals and survivors (that had been previously challenged with GL-261luc:IL-12 in the contralateral hemisphere) with GL-261luc:Fc cells; (left) representative BLI at days 1, 7, and 21; (right) BLI quantification (n ≥ 7/group). (D) Survival of WT and Ifng^−/− mice (n ≥ 10/group) injected with GL-261luc:IL-12 (E) Survival of WT and Prf1^−/− mice (n ≥ 13/group); p(Prf1^−/− vs. WT) = 0.0005. (F and G) WT mice were implanted i.c. with GL-261luc:Fc or GL-261luc:IL-12 and analyzed for TILs at day 21 using flow cytometry. (F) Representative contour plot (GL-261luc:Fc TILs gated on live CD45^hi/CD11b⁻/NK1.1⁻ cells). (G) Number (left) of CTLA-4 positive cells and CTLA-4 mean fluorescent intensity (MFI, right) for live CD4⁺FoxP3⁺, CD4⁺FoxP3⁻, or CD8⁺ T cells (n ≥ 8/group). One-way ANOVA with Bonferroni post-test; pooled data from two to three independent experiments each; *, P < 0.05; **, P < 0.01; ***, P < 0.001.