Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jul 2;1(4):206–222. doi: 10.1002/mgg3.24

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 The Author. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

PMC Copyright notice

(A) Viability of HeLa cells transfected with the mutant ATP1A2 cDNA (E825K) normalized to the viability of cells transfected with the ouabain-resistant wild-type ATP1A2 cDNA (WT). NT, untransfected cells; pcDNA3.1, cells transfected with the empty vector. Four independent experiments were performed, each with triplicate measurements. The * symbol indicates the existence of significant differences between the p.Glu825Lys and the WT ATP1A2 constructs (P < 0.0003). (B) Western blot assay of the different protein extracts using anti-myc and anti-tubulin antibodies. The molecular weight of the Na⁺/K⁺-ATPase α2 subunit and tubulin are indicated. The constructs with the ATP1A2 ouabain-resistant cDNA carry the myc tag. The clone with the mutation displayed diminished band intensity.