Table 3.
Detection sensitivity of CPDC assay.
| DNA concentration | EHEC serovar O111, GTC14517 (stx1) | EHEC serovar O157, GTC14513 (stx2) | Shigella dysenteriae serovar 2, GTC01929 (ipaH) | Salmonella enterica serovar Enteritidis GTC03838 (invA) |
|---|---|---|---|---|
| 2 ng/assay | + | + | + | + |
| 200 pg/assay | + | + | + | + |
| 20 pg/assay | + | + | + | + |
| 2 pg/assay | + | + | + | + |
| 200 fg/assay | + | + | + | + |
| 20 fg/assay | − | − | − | − |
Serially diluted purified DNA of each strain was used to count detection sensitivity.