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. 2008 Feb 25;12(4):1272–1283. doi: 10.1111/j.1582-4934.2008.00294.x

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© 2008 The Authors Journal compilation © 2008 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

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Fig. 1 — Effect of G-CSF on cardiac morphology 4 weeks after MI. (A) Transverse sections of infarcted hearts. Masson's trichrome staining. Bars, 1 mm. (B) Histological (Masson's trichrome, Sirius red) and immunohisto-chemical (Flk-1, CD45) staining of the infarcted area of a saline-treated and G-CSF-treated heart. The most right panels show negative control sections for immunostains. Arrows indicate immunopositive cells. Bars, 50 μm in CD45 staining; 100 μm in the others. (C) Haematoxylin and eosin and Flk-1 immunohistochemical staining of the border zones between infarcted and non-infarcted area of a saline-treated and G-CSF-treated heart. Arrows, arterioles; arrowheads, venules. Bars, 100 μm. (D) Histological staining (haematoxylin and eosin and Sirius red) of the non-infarcted area of hearts. Bars, 100 μm. (E) Ultrastructure of salvaged cardiomyocytes. Mf, myofibrils; Mt, mitochondria. Bars, 1 μm.

Fig. 1 — Effect of G-CSF on cardiac morphology 4 weeks after MI. (A) Transverse sections of infarcted hearts. Masson's trichrome staining. Bars, 1 mm. (B) Histological (Masson's trichrome, Sirius red) and immunohisto-chemical (Flk-1, CD45) staining of the infarcted area of a saline-treated and G-CSF-treated heart. The most right panels show negative control sections for immunostains. Arrows indicate immunopositive cells. Bars, 50 μm in CD45 staining; 100 μm in the others. (C) Haematoxylin and eosin and Flk-1 immunohistochemical staining of the border zones between infarcted and non-infarcted area of a saline-treated and G-CSF-treated heart. Arrows, arterioles; arrowheads, venules. Bars, 100 μm. (D) Histological staining (haematoxylin and eosin and Sirius red) of the non-infarcted area of hearts. Bars, 100 μm. (E) Ultrastructure of salvaged cardiomyocytes. Mf, myofibrils; Mt, mitochondria. Bars, 1 μm.