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Independent validation of down-regulated genes by real-time RT-PCR. *P < 0.05 compared with wild-type ischaemia/reperfusion group, *P < 0.05 compared with WT ischaemia/reperfusion, #P < 0.05 compared with WT preconditioning, †P < 0.05 compared with KO ischaemia/reperfusion. WTIR, wild-type IR; WTPCIR, preconditioned wild-type; KOIR, Flk-1+/− knockout IR; KOPCIR, preconditioned Flk1+/− knockout.
