Fig. 2.

Identification of MLCK minimal promoter region. Progressive increasing 5′ deletion constructs of full-length (FL) MLCK promoter were generated. Filter-grown Caco-2 cells were transfected with these constructs and promoter activities were determined by luciferase assay as described in the Materials and methods section. Data are represented as mean of four replicates ± S.E. *P< 0.05 compared to control. **P < 0.05 compared to MLCK −313 to +118.