Fig. 7.

Effect of p53 mutation on TNF-α-induced increase in MLCK promoter activity. (A) TNF-α effect on MLCK -313 promoter activity. Caco-2 cells were transfected with MLCK −313, promoter activity was determined by the luciferase assay and expressed as relative luciferase activity as described in the Materials and methods section. Data are represented as mean of three replicates ± S.E. *P< 0.05 compared to WT. (B) TNF-α effect on promoter activity of MLCK −313 with a mutated p53 binding site. Data are represented as mean of four replicates ± S.E. *P < 0.05 compared to p53 Mu.