Fig. 1.

Concentration and time dependency of particle uptake. (A) 18-hr incubation of primary human hepatocytes (24-hr pre-culture period) with increasing concentrations of MPIOs (10, 20, 30 and 40 particles/cell) resulted in an increase in particle uptake and labelling efficiency (80.00 ± 7.33%, 94.00 ± 2.79%, 96.00 ± 1.06%, 100%, respectively). (B) Time dependency of the particle uptake was investigated at a concentration of 30 MPIOs/cell (18-hr pre-culture period). Labelling efficiency: 83.00 ± 5.48%, 98.00 ± 1.85%, 99.00 ± 0.76%, 100%. Data are given as mean ± S.E.M.