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. 2013 Dec 17;2:e01426. doi: 10.7554/eLife.01426

Figure 1. Experimental design and summary of recombination events within 62 recombinants.

(A) 13 complete tetrads were generated by crossing qrt1 in a Col background to qrt1 in a Ler background, and then using single pollen tetrads from the F1 hybrids to fertilize a Cvi male sterile pollen receptor. Tetrad offspring are heterozygous, with one recombinant Col-Ler genome and one Cvi genome. 10 DH lines were generated by crossing wildtype Col-Ler F1 hybrids to the GFP-tailswap haploid inducer. One round of selfing generated doubled haploids. (B) Example of graphical genotypes of all five Col-Ler recombinant chromosomes of all four offspring of a complete tetrad. Col regions are shown in red, Ler regions in blue. The homologous chromosome, which is inherited from Cvi, is not shown. The three enlarged regions show a CO–GC, a CO without GC and a NCO–GC (clockwise, starting at the upper left corner). (C) All recombination events identified in this study. Different recombination types are labeled with different colors. Centromere positions are indicted with black lines. (D) Example of the graphical genotypes of the five chromosomes of one DH line (Col regions are shown in red, Ler regions in blue). The two enlarged regions show a NCO–GC (left) and a CO without GC (right).

DOI: http://dx.doi.org/10.7554/eLife.01426.003

Figure 1.

Figure 1—figure supplement 1. Graphical genotypes of all 13 complete tetrads.

Figure 1—figure supplement 1.

For each tetrad, all five chromosomes of all four offspring are shown (chromosome one to five, top to bottom). The four offspring genomes of one tetrad are plotted one above the other. Black lines depict peri-centromeric regions. The upper five tetrads (29, 38, 40, 58, and 62) are the deeply sequenced tetrads and were used for the detection of NCO–GCs.
DOI: http://dx.doi.org/10.7554/eLife.01426.004
Figure 1—figure supplement 2. Graphical genotypes of 10 DH lines.

Figure 1—figure supplement 2.

For each DH line, all five chromosomes are shown, chromosome one to five (top to bottom). Black lines depict peri-centromeric regions.
DOI: http://dx.doi.org/10.7554/eLife.01426.005