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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol. 2013 Nov 13;191(12):10.4049/jimmunol.1301587. doi: 10.4049/jimmunol.1301587

Figure 3.

Figure 3

The kinetics or extent of TCR signaling is altered in CD4 hAPBTs that lack FAK. A, CD4 hAPBTs were transduced with a lentivirus containing miRNAs against Luciferase (Luc) or FAK. The cells were then stimulated by soluble anti-CD3 and anti-CD4 cross-linking for various times. The site-specific phosphorylation of LAT Y191, SLP-76 Y128, and AKT S473 was analyzed by immunoblotting. The expression of FAK, Pyk2, and actin was also examined. B, Percent phosphorylation for LAT Y191, SLP-76 Y128, and Akt S473 relative to the Luciferase microRNA-transduced cells is depicted. Data are shown as mean of three experiments ± s.e.m. *p ≤ 0.05; **p ≤ 0.01.