Skip to main content
. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol. 2013 Nov 13;191(12):10.4049/jimmunol.1301587. doi: 10.4049/jimmunol.1301587

Figure 5.

Figure 5

Recruitment of Csk to membrane and TCR complex is impaired in FAK-deficient Jurkat T cells. A, Cells were stimulated on anti-TCR coated glass chamber slides for various times and stained with antibodies to detect ZAP-70 phospho-tyrosine 319 and total Csk. Cells were also stained with secondary antibodies alone (US) as a negative control. The images are representative of four independent experiments. The white scale bar is equal to 5 μm. B, For every experiment, twenty-five cells from each timepoint were analyzed using the Fiji analysis software. The intensity of Csk and ZAP-70 phospho-tyrosine 319 staining from 100 cells analyzed from four independent experiments was plotted. The horizontal line depicts the mean value (top graphs). The Manders correlation co-efficients were used to calculate Csk and phospho-ZAP-70 Y319 co-localization. The Manders co-efficients from 100 total Jurkat cells analyzed from four independent experiments were plotted. The horizontal line represents the mean value (bottom graphs). The p-values reflect the statistical differences between the control and FAK-deficient cells at the indicated times. n.s. means p > 0.05.