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. 2014 Jan 1;141(1):51–62. doi: 10.1242/dev.099382

Fig. 1.

Fig. 1.

The coiled-coil domain and the NHL domain are both required to promote the asymmetric segregation of Brat in mitotic neuroblasts. (A) Schematics of Brat and the Brat deletion mutants used to identity the domain required to asymmetrically segregate Brat in mitotic neuroblasts in transgenic Drosophila and to map the Mira-binding domain in yeast two-hybrid analysis. Dashed lines indicate deletions. (B-C′) Brat and BratΔB-boxes are cortically localized and segregate asymmetrically into the future immature INP in mitotic type II neuroblasts in brat null brains. (D-E′) Deletion of either the coiled-coil or the NHL domain (BratΔC-coil or BratΔNHL) results in cytoplasmic localization and symmetric segregation into both daughter progeny. All Brat transgenic proteins are Myc tagged. The segregation pattern of the Brat transgenic proteins was determined based on the colocalization of the Myc epitope and Mira in telophase neuroblasts. Wor> is a neuroblast-specific driver. Phh3, phosphohistone H3. (F) Yeast two-hybrid analysis showing that Brat and BratΔB-boxes interact with both Mira369-506 and Mira527-638, but BratΔC-coil only interacts with Mira527-638. (G) Summary of the domains required for segregating Brat uniquely into the future immature INP during the asymmetric division of neuroblasts. Scale bar: 5 μm.