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Loss of germline stem cells in chic mutants. (A-C′) Larval testis tips from (A-A′) wild-type, (B-B′) chic11/chic1320 hypomorph, (C-C′) chic221/chic1320 strong loss-of-function animals with anti-Arm/β-catenin (blue) to mark hub cells (asterisk), anti-Vasa (green) to mark germ cells and anti-Tj (red) to mark early cyst cell nuclei. Arrows indicate Vasa-positive cells touching the hub that were scored as GSCs. Arrowhead indicates differentiating spermatocytes. Scale bar: 20 μm.
