Fig. 2.

chic is cell autonomously required for maintenance of germline stem cells. (A) Percentage of testes with at least one GFP-negative, homozygous control FRT40A (blue), chic²²¹ (red) or chic^P5205 (green) GSC over time after clone induction. Numbers next to data points reflect the number of testes scored for each genotype at each time point. Error bars indicate s.e.m. See also supplementary material Fig. S1. (B-G′) Adult testis tips from (B,E-E′) wild type, (C,F-F′) from nanos-GAL4/UAS-chicRNAi resulting in knockdown of chic in the germ line and from (D,G-G′) chic²²¹/chic¹³²⁰ strong loss-of-function mutant. (B-D) Phase-contrast images. White arrowheads indicate elongated spermatid bundles. Scale bar: 10 μm. (E-G′) Anti-Vasa to mark germ cells. Anti-Arm to mark hub cells. Yellow arrowheads indicate GSCs abutting the hub. Scale bar: 20 μm.