chic is required in germ cells for E-cadherin localization to the hub-GSC interface. (A-Ba′′) Larval testes expressing E-cad-GFP in early germ cells. (A-Aa′′) +/+; nanos-GAL4/UAS-Ecad-GFP controls, (B-Ba′′) chic11/chic1320; nanos-GAL4/UAS-Ecad-GFP animals. Anti-GFP (green) to highlight expression of the E-cad-GFP transgene, anti-Vasa (red) and anti-Arm (blue) to outline hub cells, and anti-Tj (blue) to mark somatic cyst cells. Yellow arrowheads indicate E-cadherin-GFP localized to hub-GSC interface. Scale bars: 20 μm in A-A′′,B-B′′; 10 μm in Aa-Aa′′,Ba-Ba′′.