Figure 4.

Bone marrow-derived mesenchymal stromal cells preactivated with mast cells induce proliferation of CD34⁺ progenitor cells and regulate allergy-related genes in CD34⁺ progenitors. CFSE-labeled freshly isolated CD34⁺ progenitor cells were co-cultured for 3 days with BM-MSCs exposed to activated in a Transwell system mast cells (kept in cultures for 6 h while cross-linking with anti-IgE and then removed) and their proliferation was assessed. Numbers refer to mean fluorescence intensity (MFI) of CFSE-labeled CD34⁺-gated cells. Representative of three experiments.