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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: Clin Cancer Res. 2011 Sep 20;17(22):10.1158/1078-0432.CCR-11-1899. doi: 10.1158/1078-0432.CCR-11-1899

Fig. 1. Modification of Kozak sequence of GLIPR1/RTVP-1 cDNA improves GLIPR1 protein expression.

Fig. 1

Unmodified and modified GLIPR1/RTVP-1 cDNA constructs were transfected in A459 cell line and cell lysates were analyzed by western blot for GLIPR1 protein expression levels 48 hour after transfection. Lanes: 1. pcDNA3.1 (control vector); 2. pShuttle-hRTVP-1: 3. pSecTag-hRTVP-1; 4. pShuttle-hRTVPk1, 5. pShuttle-hRTVPk2, 6. pShuttle-hRTVPk3. All three pShuttle-hRTVPk clones with modified Kozak sequence (lane 4–6) demonstrated increased expression of GLIPR1/hRTVP-1 protein compared to the original pShuttle-hRTVP-1 vector (lane 2).