Figure 1.

The impact of DO inhibition of DM on the MHCII-bound peptide repertoire. Immature DCs and resting B cells (bottom) express high levels of DO and thus have pools of active endosomal DM as well as inactive DM-DO complexes that shape the overall peptide repertoire. The net result is a broad, diverse set of lower stability peptides presented on MHCII. Self-peptides (red) including CLIP (black peptide) are well represented. Activated DCs and B cells and other DO-negative APC (top) are enriched for DM due to DO downregulation and thus display a more focused and high stability peptide repertoire. Self-peptide presentation including CLIP is reduced at the expense of high stability peptide presentation promoted by fully active DM editing.