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. Author manuscript; available in PMC: 2014 Oct 12.
Published in final edited form as: Circ Res. 2013 Jul 29;113(9):10.1161/CIRCRESAHA.113.301340. doi: 10.1161/CIRCRESAHA.113.301340

Figure 1. Generation of a floxed allele of the mouse Synectin gene.

Figure 1

(A) The targeting vector contains: a loxP site (black triangle) into BsrB I restriction site upstream of exon 2, exon 2 cDNA ,a fragment with Neo selection cassette (grey arrow) and a loxP site into the PshAI site downstream of exon 2. The Frt-flanked Neo-cassette was removed by Flp-mediated, site-specific recombination (Frt recombination elements are white triangles). (B) Genomic mouse DNA was genotyped with the oligonucleotides indicated in A (black arrows).