Figure 3.

UV dose-dependence of chromosomal fragmentation in recBC(Ts) or its derivatives harboring additional mutations in polA or ligA genes. Agarose plugs were made after incubating the UV-exposed cultures at 37°C for 10 minutes. Plugs were run either at 6 V/cm for 24 H or at 3 V/cm for 72 H as described in Fig. 1. Panels A, C and E are radiograms of the gels. Panels B, D and F show quantification of fragmentation from the corresponding gels. CZ, compression zone.