Skip to main content
. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Anal Biochem. 2013 Jun 14;443(2):10.1016/j.ab.2013.06.001. doi: 10.1016/j.ab.2013.06.001

Figure 6.

Figure 6

The effect of nicks on the quantity of NotI-digested sub-chromosomal fragments. Stationary cultures of recBC(Ts) and recBC(Ts) uvrA mutants were exposed to a variety of UV doses, and plugs were made after 10 min incubation in spent culture medium. One set of plugs was digested with NotI and electrophoressed, together with non-NotI digested plugs, at 6 V/cm for 20 H and 3 V/cm for 70 H.

(A) Quantification of the amount of signal, presented as % of the total signal, in 1 Mbp NotI fragment after electrophoresis at 6 V/cm for 20 H.

(B) The radiogram of the gel from which the data in (A) are derived.

(C) Qauntification of the amount of signal, presented as % of the total signal, in the compression zone after electrophoresis at 3 V/cm for 70 H.

(D) The radiogram of the gel from which the data in (C) are derived.