Table 1.
Strains used in this study.
| Strain | Relevant genotype | Source or reference | Properties |
|---|---|---|---|
|
| |||
| AB1157 | wild-type strain | [58] | Repair proficient |
| SK129 | recB270(Ts) recC271(Ts) | [59] | Defective in DSB repair and DNA degradation |
| AK107 | SK129 dut-1 zic4901::Tn10 | [38] | Defective in DSB repair and also incorporates uracil in DNA |
| AK25 | AB1157 polA12(Ts)::Tn10 | Lab Collection | Defective in excision DNA repair |
| GR501 | GR523 ligA251(Ts) | [60] | Defective in sealing DNA nicks |
| LA20 | GR501 ypeB::kan | [61] | Defective in sealing DNA nicks |
| SRK312 | SK129 polA12(Ts)::Tn10 | SK129 × P1 AK25 | Defective in DSB repair and excision repair |
| SRK322 | SK129 ligA251(Ts) | SK129 × P1 LA20 | Defective in DSB repair and sealing DNA nicks |
| SRK301 | SK129 ΔuvrA277::Tn10 | [5] | Defective in DSB- and nucleotide excision repair |