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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: Nat Cell Biol. 2013 Apr 21;15(5):10.1038/ncb2731. doi: 10.1038/ncb2731

Figure 1.

Figure 1

FBXL2 binds p110-free p85 regulatory subunits. (a) FBXL2 binds p85α and p85β. HEK293T cells were transfected with either an empty vector (EV) or the indicated FLAG-tagged F-box proteins (FBPs). 24 h post-transfection, cells were treated with MG132 for 3 h before collection for immunoprecipitation (IP) and immunoblotting as indicated. WCL, whole-cell lysate. (b) FBXL2 does not bind p110α and p110β. HEK293T and NIH3T3 cells were transfected with either an empty vector (EV) or the indicated FLAG-tagged proteins. The experiment was performed as described in a. (c) The CaaX motif of FBXL2 is required to bind p85α and p85β. HEK293T cells were transfected with either an empty vector (EV) or the indicated FLAG-tagged F-box proteins. The experiment was performed as described in a. (d) Table summarizing the results presented in a–c and Supplementary Fig. S1a. NT, not tested. Uncropped images of blots are shown in Supplementary Fig. S8.