Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description | Reference or source | |
|---|---|---|---|
| S. cerevisiae | |||
| InvSc1 | Diploid uracil auxotroph | Invitrogen | |
| Bacterial strains | |||
| S17-1 λpir | Escherichia coli strain used for conjugation | [22] | |
| EC100D | E. coli strain used for cloning, pir-116 | Epicentre | |
| ER2566 | E. coli strain used for protein expression | New England Biolabs | |
| CMS376 | wild-type Serratia marcescens, PIC strain number 3611 | Presque Isle Cultures | |
| CMS1779 | hexS::pStvz3 in WT (CMS376), hexS-1 | This study | |
| CMS2210 | ΔhexS, (hexS-Δ1 allele) in CMS376 | This study | |
| CMS3125 | CMS2210 + pMQ294 hexS complementation strain | This study | |
| CMS3142 | nuoC::pStvZ3 in CMS376 | This study | |
| CMS1317 | alaT::pMQ118 in CMS376 | This study | |
| Plasmids | |||
| pMal-C2 | Maltose binding protein fusion construct | New England Biolabs | |
| pStvZ3 | oriR6K lacZ nptII promoter probe | [20] | |
| pMQ118 | suicide vector nptII, rpsL, oriT, URA3, CEN6/ARSH4 | [19] | |
| pMQ197 | pMQ118 with internal fragment of alaT | This study | |
| pMQ236 | allelic replacement vector, pMQ118 with I-SceI site | [19] | |
| pMQ240 | I-SceI-expression plasmid, aacC1, oripSC101ts | [19] | |
| pMQ268 | pStvZ3 + pigA internal fragment | [20] | |
| pMQ294 | pMQ236 with hexS open reading frame | This study | |
| pMQ296 | pMQ294 with hexS-Δ1 mutant allele | This study | |
| pMQ342 | pStvZ3 with internal fragment of nuoC | This study | |
| pMQ402 | pMAL-C2 + hexS (MBP-HexS fusion construct) | This study |