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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: Biochem J. 2012 Sep 1;446(2):10.1042/BJ20120768. doi: 10.1042/BJ20120768

Figure 2. Silencing of PRMT5 expression inhibits lung cancer cell growth.

Figure 2

(A) Short hairpin RNA (shRNA)-mediated silencing of PRMT5 expression in lung cancer cells shown by Western blot analysis of whole cell lysates from A549 cells infected with a lentivirus expressing nontargeting (NT) shRNA (lane 1), PRMT5 shRNA (lane 2), PRMT5 shRNA plus shRNA-resistant PRMT5 (lane 3), and PRMT5 shRNA plus shRNA-resistant mutant PRMT5 (PRMT5mt; lane 4). (B) Growth curves of lung cancer cells expressing NT shRNA, PRMT5 shRNA, PRMT5 shRNA plus shRNA-resistant PRMT5, and PRMT5 shRNA plus shRNA-resistant PRMT5mt. (C) A549 cells infected with NT shRNA- or PRMT5 shRNA-expressing lentivirus were allowed to grow in the presence of BrdU and immunostained with an anti-BrdU antibody (brown). The white arrow (left panel) indicated the BrdU-negative staining cell and the black arrows (right panel) indicated the BrdU-positive staining cells. Original amplification is 200 and the scale bar represents 10 μm.