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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: J Endocrinol. 2007 Apr;193(1):10.1677/joe.1.06927. doi: 10.1677/joe.1.06927

Figure 2.

Figure 2

Expression of POMC(A and B), convertases PC1 and PC2 (D and E) mRNAs and ACTH (C) in ARPE-19 cells. (A and B) Two different sets of primers were used for RT-PCR detection of fragments of POMC mRNA as published (Slominski et al. 1995, 2005). (C) Immunocytochemical detection of ACTH in human ARPE cells of passages 24 (p24) and 30 (p30). Colour panels and control experiments are shown in supplementary Fig. 1 (http://joe.endocrinology-journals.org/content/vol193/issue1/).Bar, 25 µm. RT-PCR detection of prohormone convertases: PC1, 674 bp (D) and PC2, 299 bp (E). Primers and RT-PCR conditions are described in Materials and Methods section. On panels (A, B, D and E):DNA ladder, M; ARPE-19 passages 24, p24; and 30, p30; brain (positive control), B and negative control without cDNA, C.

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