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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: J Endocrinol. 2007 Apr;193(1):10.1677/joe.1.06927. doi: 10.1677/joe.1.06927

Figure 5.

Figure 5

CRF stimulates transcriptional activity of CRE (A) and AP1 responsive elements (B) and POMC promoter (C). ARPE-19 cells, passages 25–28, were transiently transfected with firefly luciferase reporter gene constructs: pCRE-Luc (under control of CRE), pAP1-Luc (under control of AP1) and p-POMC-Luc (driven by the sequence of the human POMC promoter −771 to −8) and phRL-TK (Renilla luciferase used as transfection efficiency control; Pisarchik & Slominski 2004, Slominski et al. 2005, Zbytek et al. 2006). The transiently transfected cells were treated with serial dilutions of CRF, lysed after 24 h after treatment and activity of the promoter measured. Data are presented as means±s.e.m. (n=3). *P<0.05.

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