Table 1.
Sequences of primers
| Primer |
Primer location |
Fragment size |
Reference |
|
|---|---|---|---|---|
| Gene | ||||
| CRF | MZ024 5′-CACCCTCAGCCCTTGGATTTC-3′ | Exon 2 | 413 bp | Slominski et al. (1995) |
| MZ025 5′-GCCCTGGCCATTTCCAAGAC-3′ | Exon 2 | |||
| Ucn I | MZ026 5′-CAGGCGAGCGGCCGCG-3′ | Exon 2 | 145 bp | Bamberger et al. (1998) |
| MZ027 5′-CTTGCCCACCGAGTCGAAT-3′ | Exon 2 | |||
| Ucn II | MZ103 5′-GTGTCGGCCACTGCTGAGCCTGAGAGA-3′ | Exon 2 | 195 bp | Imperatore et al. (2006) |
| MZ104 5′-ATCTGATATGACCTGCATGACAGTGGCT-3′ | Exon 2 | |||
| Ucn III | P575 5′-TGAGCAAGAGGAGCTTCCAC-3′ | Exon 1 | 318 bp | * |
| P576 5′-TCCTCCCAATTTGCGCCATC-3′ | Exon 1 | |||
| CRFR1 | CRFR1 fragment spanning exons 2–7 | Pisarchik & Slominski (2001) | ||
| First pair of primers | Exon 2 | PCR fragments after second round of amplification | ||
| P110, 5′-TCCGTCTCGTCAAGGCCCTTC-3′ | Exon 7 | 479 bp Insertion of cryptic exon between exons 4 and 5 (CRFR1h) | ||
| P111, 5′-GGCTCATGGTTAGCTGGACCAC-3′ | 369 bp (absent exon 6 CRFR1α, d, f, g) | |||
| Nested primers | 249 bp Exons 3 and 6 are absent (CRFR1c) | |||
| P112, 5′-TGTCCCTGGCCAGCAACATCTC-3′ | Exon 2 | 163 bp exons 3, 4 and 6 are absent (frameshift, CRFR1e) | ||
| P113, 5′-AGTGGATGATGTTTCGCAGGCAC-3′ | Exon 7 | |||
| CRFR1 fragment spanning exons 9–14 | PCR fragments after second round of amplification | Pisarchik & Slominski (2001) | ||
| First pair of primers | 336 bpAll exons are present (CRFR1α, β, c) | |||
| P114 5′-CCATTGGGAAGCTGTACTACGAC-3′ | Exon 9 | 294 bp Exon 13 is absent (CRFR1d) | ||
| P115 5′-GCTTGATGCTGTGAAAGCTGACAC-3′ | Exon 14 | 200 bp Exon 12 is absent (frameshift, CRFR1f) | ||
| Nested primers | 114 bp Exon 11, 27 bp of exon 10 and 28 bp of exon 12 are absent (CRFR1g) | |||
| P116 5′-GGGTGTACACCGACTACATCTAC-3′ | Exon 9 | |||
| P117 5′TCTTCCGGATGGCAGAACGGAC-3′ | Exon 14 | |||
| POMC | POMC gEfor 5′-GAGGGCAAGCGCTCCTACTCC-3′ | Exon 3 | 261 bp | Slominski et al. (1995) |
| POMC gErev 5′-GGGGCCCTCGTCCTTCTTCTC-3′ | Exon 3 | |||
| POMC | POMC PXfor 5′-CTACGGCGGTTTCATGACCT-3 | Exon 3 | 100 bp | Slominski et al. (2005) |
| POMC PXrev 5′-CCCTCACTCGCCCTTCTTG-3′ | Exon 3 | |||
| CYP11A1 | First pair of primers | Slominski et al. (2004c) | ||
| NM_000781 | P561 5′-GCCTTTGAGTCCATCACTAAC-3′ | Exon 4 | 628 bp | |
| P562 5′-CCAGTGTCTTGGCAGGAATC-3′ | Exon 7 | |||
| Nested Primers | 390 bp | |||
| P563 5′-ATGTGGCTGCATGGGACGTG-3′ | Exon 4 | |||
| P564 5′-TCTGCAGGGTCACGGAGATG-3′ | Exon 7 | |||
| CYP11B1 | MZ127 5′-CTGGGACATTGGTGCGC-3′ | Exon 8/9 | 215 bp | Lin et al. (2006) |
| MZ128 5-GTGTTTCAGCACATGGT-3′ | Exon 9/10 | |||
| CYP17 | First pair of primers | 251 bp | * | |
| NM_000102 | P565 5′-CTCTAGACATCGCGTCCAAC-3′ | Exon 2 | ||
| P566 5′-GAAGCAGATCAAGGAGATGAC-3′ | Exon 3 | |||
| Nested Primers | 222 bp | |||
| P587 5′-TCGCGTCCAACAACCGTAAG-3′ | Exon 2 | |||
| P588 5′-CATTGGTTACCGCCACGAAG-3′ | Exon 3 | |||
| CYP21A2 | First pair of primers | 299 bp | * | |
| NM_0005002 | P593 5′-CTGAAGCAGGCCATAGAGAAG-3′ | Exon 7 | ||
| P594 5′-AGTTCGTGGTCTAGCTCCTC-3′ | Exon 8 | |||
| Nested primers | 199 bp | |||
| P396 5′-TGGAGGGACATGATGGACTAC-3′ | Exon 7 | |||
| P397 5′-CCTGCAGTCGCTGCTGAATC-3′ | Exon 8 | |||
*
refers to current paper.