. Author manuscript; available in PMC: 2013 Dec 17.

Published in final edited form as: Bioelectrochemistry. 2009 Mar 20;76(0):10.1016/j.bioelechem.2009.03.007. doi: 10.1016/j.bioelechem.2009.03.007

Table 1.

Effect of co-adsorbate on E-DNA signaling, specificity and reusability.

Thiol

Chemical formula

Initial signal/μA^a

(I_target/I_buffer)/% T-17^b

(I_target/I_buffer)/% T-27^b

(I_target/I_buffer)/% M-17^b

Specificity ratio Rs^c

Regeneration (I_reg/I_buffer)/%^d

2-mercaptoethanol

3.9±0.3

62±3

70±4

50±3

1.2

85±5

6-mercaptohexanol

HO - {({CH}_{2})}_{6} - SH

4.7±0.5

71±4

80±3

55±3

1.3

98±6

11-mercaptoundecanol

HO - {({CH}_{2})}_{11} - SH

4.5±0.4

47±3

60±4

25±2

1.9

96±8

Cysteamine (2-mercaptoethylamine)

HO - {CH}_{2} - {CH}_{2} - {NH}_{3}^{+}

5.9±0.7

90±6 ^d

90±4

77±5

1.1

89±6

Mercaptoethane sulfonic acid

graphic file with name nihms524203t2.jpg

2.6±0.4

80±5

52±3

36±6

2.2

91±7

Signal in the presence of buffer.

% values of signal suppression (ratio of current signal obtained in target–I_target–and current signal obtained in buffer -I_buffer) as a result of target hybridization are the average and standard deviation of measurements performed with four independent sensors.

R_s is the ratio of the signal suppression (I_target/I_buffer) obtained with the fully complementary target (T-17) to that observed with the mismatched target (M-17).

% values of regeneration (ratio of current signal obtained in buffer after regeneration–I_reg–and current signal obtained in buffer–I_buffer) are the average and standard deviation of measurements performed with four independent sensors.